Analysis of the Y chromosome in men with azoospermia or severe oligozoospermia has resulted in the identification of three regions in the euchromatic part of the long arm of the human Y chromosome (Yq11) that are frequently deleted in men with otherwise unexplained spermatogenic failure.

PCR analysis of micro deletions in the AZFa, AZFb and AZFc (AZF: Azoospermia Factor) regions of the human Y chromosome is an important screening tool in the work-up of infertile males opting for assisted reproductive techniques. Y chromosome micro deletions are the most common genetic cause of male infertility and screening for these micro deletions in azoospermic or severely oligospermic men is now standard practice in many infertility centers.

Diagnostic testing using the Devyser AZF kits relies on PCR amplification of sequence-tagged sites (STS) in the AZFa, AZFb and AZFc regions on the Y-chromosome followed by analysis by capillary electrophoresis. Successful amplification of an STS marker indicates presence, whereas absence of PCR amplification is indicative of deletion.

Devyser’s unique single-tube approach simplifies the workflow, reduces hands-on time and minimizes the risk of sample mix-up and contamination during both basic and extended AZF analysis.