Testing according to EAA and EMQN best practice guidelines
All STS markers and control sequences, recommended by the European Academy of Andrology (EAA) and the European Quality Monitoring Network Group (EMQN) for basic molecular diagnosis of Y-chromosomal microdeletions in the AZFa, AZFb and AZFc regions are included in the kit. If a suspected deletion is detected an extended AZF marker analysis should be done using the Devyser AZF Extension kit.
The use of fluorescently labelled primers for all markers allows automated visualization and identification of the STS markers using a Genetic Analyzer. Thus, eliminating multiple PCR reactions, use of toxic chemicals associated with the use of EtBr stained Agarose gels, and the guesswork associated with fragment identification
AZF testing kits
Diagnostic testing using Devyser AZF v2 relies on PCR amplification of sequence-tagged sites (STS) in the AZFa, AZFb and AZFc regions on the Y-chromosome. Successful amplification of an STS marker indicates presence, whereas absence of PCR amplification is indicative of deletion.
Devyser AZF v2 – for basic deletion testing
Devyser AZF v2 allows detailed analysis of Y-chromosome microdeletions in the AZFa, AZFb and AZFc regions. Conforms to the EAA and EMQN best practice guidelines for basic molecular diagnosis of Y-chromosomal microdeletions. Combine with Devyser AZF Extension for a complete basic and extended workflow
Conduct extended molecular diagnosis of Y-chromosomal microdeletions in the AZF region using Devyser AZF Extension. Conforms to the EAA and EMQN best practice guidelines for extended molecular diagnosis of Y-chromosomal microdeletios