Devyser's NGS kit combines high sensitivity with accurate measurement and enables very early detection of graft rejection and disease relapse in stem cell transplanted patients.
Accurate measurement down to 0.05% minority fraction
Less than 45 minutes hands-on time
Fast and easy workflow from patient sample to report
Devyser Chimerism for NGS is a CE-IVD kit that provides your lab with one simple protocol for fast and reliable chimerism measurement and monitoring in transplanted patients. Complete relapse monitoring is possible with high sensitivity, accurate measurement and a custom software that makes it easy to follow chimerism trends in patients.
Chimerism analysis using NGS enables high sensitivity and accurate measurement throughout the dynamic range. This is achieved by combining the sensitive analysis of real-time PCR with the accurate measurements at high chimerism levels of STR fragment analysis, as illustrated in these two diagrams.
The first diagram shows high accuracy and correlation between Devyser Chimerism for NGS and STR fragment analysis at high chimerism levels (more than >1% of minority fraction). It is also clear that real-time PCR measurements are generally not accurate at chimerism levels above 20% of minority fraction.
The second diagram, a magnified view of the low end of the range, shows that STR fragment analysis has its limitations to correctly measure minority fraction below 3-5%. However, Devyser Chimerism for NGS and real-time PCR show high accuracy and correlation at low level minority fraction.