QF-PCR and maternal cell contamination
QF-PCR and maternal cell contamination by Dr Kathy Mann
The ability to identify maternal cell contamination (MCC) in uncultured AF and CV samples minimises the risk of misdiagnosis due to the analysis of maternal cells. Authored by Dr. Kathy Manns, the pioneer in prenatal QF-PCR analysis, this paper provides information for laboratory professionals on how to identify the presence and level of MCC in a prenatal sample using a QF-PCR based approach.
What you'll learn
- Detection of MCC by QF-PCR
- The benefit a QF-PCR based approach has over other methods
- Best practices for handling heavily blood-stained AF samples
- Interpretation of the results
Prenatal diagnosis currently involves testing material that is obtained by chorionic villus (CV) or amniocentesis sampling; either of these procedures may result in contamination of the sample with maternal cells and the potential for misdiagnosis. It is important to identify the presence and level of maternal cell contamination (MCC) in a prenatal sample as this may have implications for sample processing and result interpretation.
The removal of maternal decidua from chorionic villi (CV) samples is standard practice prior to testing and therefore MCC is rarely observed in CV samples of usual quality. However, for CV samples of poorer quality, removal of maternal material may be technically difficult; such samples may retain maternal material and give rise to MCC of uncultured and/or cultured villi.